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Maximum "clearance" can be achieved by circulating the sample
stream and the dialysate stream on different sides of the semi-permeable
membrane in opposite (counter-current) directions to provide the
largest concentration gradient and pressure differential possible.
Clearance (Cs) is defined as the overall mass transfer rate of a solute
per unit of time (ml/min) and is proportional to the feed flow rate of a
solution (Qf) and to the concentration difference across the membrane
barrier (Cbi - Cbo) less the sweep solution concentration (Cdi).
Cs = Qf(Cbi - Cbo) / Cbi - Cdi
(Note that Cdi is essentially 0 in an open system with sweep flow to drain. )
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ADVANTAGES OF DYNAMIC DIALYSIS
- Rapid and Efficient Dialysis
- Saves Valuable Time
- Dialysis of Larger Sample Volumes
- Flow Stream Prevents Membrane Fouling & Maintains Suspension of Solutes
- Eliminates Need for Dialysate Changes
- Open System: dialysate goes to drain
- Closed System: dialysate recirculated
Applications:
- Desalting or pH Change
- Plasma / Serum Concentration
- Protein Preparation
- Electrophoresis Gels
- Antibody Concentration
- Clarification and Purification
- Binding Studies
- Temperature Regulated Dialysis
- Open System & Batch Dialysis
Dialysis Resources: 
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